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Glycoproteins and protein glycations identified in barley grain and malt by 2D-HPLC

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    0391056 - ÚIACH 2013 CZ eng A - Abstract
    Žídková, Jitka - Petry-Podgórska, Inga - Laštovičková, Markéta - Bobálová, Janette
    Glycoproteins and protein glycations identified in barley grain and malt by 2D-HPLC.
    Materials Structure in Chemistry, Biology, Physics and Technology. Czech and Slovak Crystallographic Association. Roč. 20, č. 1 (2013), s. 43. ISSN 1211-5894.
    [Discussion in Structural Molecular Biology /11./. 14.03.2013-16.03.2013, Nové Hrady]
    R&D Projects: GA ČR(CZ) GPP503/12/P395
    Institutional support: RVO:68081715
    Keywords : barley grain * glycoproteins * 2D-HPLC * MS/MS
    Subject RIV: CB - Analytical Chemistry, Separation

    Two-dimensional liquid chromatography (ion-exchange and reversed-phase) with off-line mass-spectrometric detection (MALDI-TOF-MS/MS) has been used to analyze glycoproteins and glycated proteins in barley grain and malt. Seven glycoproteins were identified by partial sequencing based on fragmentation MS/MS spectra. Glycations were observed for five out of 16 unambiguously assigned proteins in malt extract, while no glycation was detected in barley grains. The results document that the proposed analytical procedure is well suited for monitoring carbohydrate-modified proteins in cereal products and their end foodstuff products.
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