Phenotyping of circulating CD8(+) T cell subsets in human cutaneous leishmaniasis

0389981 - ÚMG 2013 RIV NL eng J - Journal Article
Khamesipour, A. - Rostami, M.N. - Tasbihi, M. - Mohammadi, A.M. - Shahrestani, T. - Sarrafnejad, A. - Sohrabi, Yahya - Eskandari, S.E. - Valian, H.K.
Phenotyping of circulating CD8(+) T cell subsets in human cutaneous leishmaniasis.
Microbes and Infection. Roč. 14, č. 9 (2012), s. 702-711. ISSN 1286-4579. E-ISSN 1769-714X
Institutional research plan: CEZ:AV0Z50520514
Institutional support: RVO:68378050
Keywords : CD8(+) T cells * memory T cells * cutaneous leishmaniasis * IFN-gamma
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.920, year: 2012

Recovery from CL is usually accompanied with long-lasting protection and induction of strong immune response. The phenotypes, generation and maintenance of central (=T-CM) and effector (=T-EM) memory T cell subsets in human leishmaniasis are not well known. Profile of T cell subsets were analyzed on peripheral CD8(+) T cells from volunteers with history of cutaneous leishmaniasis (HCL). In HCL and control groups, mean frequencies of CCR7(+)CD45RA(+)CD8(+) naive and CCR7(-)CD45RA(-)CD8(+) T-EM cells were higher than other subsets before culture, but after stimulation with soluble Leishmania antigen, the frequency of naive T cells was significantly decreased and the frequency of T-EM cells was significantly increased. T-EM phenotype composed the highest portion of proliferating Carboxy Fluorescein diacetate Succinimidyl Ester (CFSE)-dim population which was significantly higher in HCL volunteers than in control group. Stimulation of isolated CD8(+) memory T cells, but not naive T cells, from HCL volunteers induced a significantly higher IFN-gamma production compared with that of healthy controls. Intracellular IFN-gamma staining provided the same result. Memory population is shown to be responsible for Leishniania-induced IFN-gamma production. Leishmania-reactive proliferating T-EM cells were identified as the most frequent subset which may play a role in recall immune response and protection against Leishmania infection.
Permanent Link: http://hdl.handle.net/11104/0218934