Impact of oxidized phospholipids on the structural and dynamic organization of phospholipid membranes: a combined DSC and solid state NMR study

0388697 - ÚFCH JH 2014 RIV GB eng J - Journal Article
Wallgren, M. - Beranová, Lenka - Pham, Q. D. - Linh, K. - Lidman, M. - Procek, J. - Cyprych, K. - Kinnunen, P. K. J. - Hof, Martin - Gröbner, G.
Impact of oxidized phospholipids on the structural and dynamic organization of phospholipid membranes: a combined DSC and solid state NMR study.
Faraday Discussions. Roč. 161, DEC 2013 (2013), s. 499-513. ISSN 1359-6640. E-ISSN 1364-5498
R&D Projects: GA AV ČR GEMEM/09/E006
Institutional support: RVO:61388955
Keywords : ACYL-CHAIN REVERSAL * MOLECULAR SIMULATIONS * LIPID-MEMBRANES
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 4.194, year: 2013

Membranes undergo severe changes under oxidative stress conditions due to the creation of oxidized phospholipid (OxPL) species, which possess molecular properties quite different from their parental lipid components. These OxPLs play crucial roles in various pathological disorders and their occurrence is involved in the onset of intrinsic apoptosis, a fundamental pathway in programmed mammalian cell death. However, the molecular mechanisms by which these lipids can exert their apoptotic action via their host membranes (e.g., altering membrane protein function) are poorly understood. Therefore, we studied the impact of OxPLs on the organization and biophysical properties of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) based lipid membranes by differential scanning calorimetry (DSC) and solid state nuclear magnetic resonance (NMR) spectroscopy. Incorporation of defined OxPLs with either a carboxyl group (1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PazePC)) or aldehyde (1-palmitoyl-(90oxononanoyl)-sn-glycero-3-phosphocholine (PoxnoPC)) at their truncated sn-2-chain ends enabled us to reveal OxPL speciesdependent differences. The calorimetric studies revealed significant effects of OxPLs on the thermotropic phase behavior of DMPC bilayers, especially at elevated levels where PazePC induced more pronounced effects than PoxnoPC.
Permanent Link: http://hdl.handle.net/11104/0217605