Optical Fiber Whole Cell Bioluminescent Sensor

0387376 - ÚCHP 2013 CA eng C - Conference Paper (international conference)
Kuncová, Gabriela - Pospíšilová, M. - Solovyev, Andrey
Optical Fiber Whole Cell Bioluminescent Sensor.
Book of Abstracts. -: -, 2012, s. 96-97. ISBN N. ISSN 1877-7058.
[International Conference on Bioencapsulation /20./. Orillia, Ontario (CA), 21.09.2012-24.09.2012]
R&D Projects: GA AV ČR(CZ) IAAX08240901
Institutional support: RVO:67985858
Keywords : whole cell biosensor * fibre sensor * Pseudomonas putida TVA8
Subject RIV: CC - Organic Chemistry

The preparation and application of the optical fiber biosensor of BTEX (benzene, toluene, ethylbenzene and xylene) with bioluminescent bioreporter Ps. Putida TVA8 entrapped into silica gel on tapered fiber was demonstrated. The sensor bioluminescence was repeatedly induced with toluene (26.5 mg/L) for 32 days. The recorded response times of bioluminescence maxima were from 1 to 12 hours. Between 7th and 18th day the response times were 1-2 hours and bioluminescence maxima from 2000-5000 cps. At least within this period the sensor worked as reliable detector of toluene.
Permanent Link: http://hdl.handle.net/11104/0216581