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Bioluminescence determination of active caspase-3 in single apoptotic cells

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    0387334 - ÚIACH 2014 RIV DE eng J - Journal Article
    Lišková, Marcela - Klepárník, Karel - Matalová, Eva - Hegrová, Jitka - Přikryl, Jan - Švandová, Eva - Foret, František
    Bioluminescence determination of active caspase-3 in single apoptotic cells.
    Electrophoresis. Roč. 34, č. 12 (2013), s. 1772-1777. ISSN 0173-0835. E-ISSN 1522-2683
    R&D Projects: GA ČR GAP206/11/2377
    Grant - others:GA ČR(CZ) GAP502/12/1285
    Program: GA
    Institutional support: RVO:68081715 ; RVO:67985904
    Keywords : apoptosis * bioluminescence * caspase-3
    Subject RIV: CB - Analytical Chemistry, Separation
    Impact factor: 3.161, year: 2013

    In this work we report on the development of a high-sensitivity instrumentation and protocol for detection of active caspase-3 in individual mammalian apoptotic cells. The technology is based on the specific cleavage of modified luciferin by caspase-3, an immediate bioluminescence reaction of free luciferin with luciferase followed by emissions of photons and their detection by photomultiplier tube working in the photon counting regime.
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