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Probing nucleic acid interactions and pre-mRNA splicing by Förster resonance energy transfer (FRET) microscopy

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    0387290 - ÚMG 2013 RIV US eng J - Journal Article
    Šimková, Eva - Staněk, David
    Probing nucleic acid interactions and pre-mRNA splicing by Förster resonance energy transfer (FRET) microscopy.
    International Journal of Molecular Sciences. Roč. 13, č. 11 (2012), s. 14929-14945. E-ISSN 1422-0067
    R&D Projects: GA AV ČR KAN200520801
    Institutional support: RVO:68378050
    Keywords : FRET * FLIM * acceptor photobleaching * RNA interactions * spliceosome
    Subject RIV: EB - Genetics ; Molecular Biology
    Impact factor: 2.464, year: 2012

    Förster resonance energy transfer (FRET) microscopy is a powerful technique routinely used to monitor interactions between biomolecules. Here, we focus on the techniques that are used for investigating the structure and interactions of nucleic acids (NAs). We present a brief overview of the most commonly used FRET microscopy techniques, their advantages and drawbacks. We list experimental approaches recently used for either in vitro or in vivo studies. Next, we summarize how FRET contributed to the understanding of pre-mRNA splicing and spliceosome assembly.
    Permanent Link: http://hdl.handle.net/11104/0218713

     
     
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