Open-tubular capillary electrochromatography with bare gold nanoparticles-based stationary phase applied to separation of trypsin digested proteins

0387211 - ÚOCHB 2013 CZ eng C - Conference Paper (international conference)
Mikšík, Ivan - Lacinová, Kateřina - Zmatlíková, Zdeňka - Sedláková, Pavla - Král, V. - Sýkora, D. - Řezanka, P. - Kašička, Václav
Open-tubular capillary electrochromatography with bare gold nanoparticles-based stationary phase applied to separation of trypsin digested proteins.
Acta Universitatis Palackianae Olomucensis. Facultas Rerum Naturalium. Chemica 50S. Olomouc: Faculty of Science, Palacký University Olomouc, 2012 - (Maier, V.; Ševčík, J.), s. 16-17. ISBN 978-80-244-3115-4. ISSN 0232-0061.
[International Symposium Advances in Chromatography and Electrophoresis and Chiranal. Olomouc (CZ), 11.06.2012-14.06.2012]
R&D Projects: GA ČR(CZ) GA203/09/0675; GA ČR(CZ) GAP206/12/0453
Institutional research plan: CEZ:AV0Z50110509; CEZ:AV0Z40550506
Keywords : open-tubular capillary electrochromatography * gold nanoparticles * glycation of proteins
Subject RIV: CB - Analytical Chemistry, Separation

Open-tubular capillary electrochromatography (OT-CEC) using bare gold nanoparticles-based stationary phase has been applied to separation of tryptic peptide fragments of native and glycated proteins, bovine serum albumin and human transferrin. OT-CEC with bare gold nanoparticles stationary phase was found to be a suitable technique for separation of complex peptide mixtures originating from enzymatic (tryptic) digestion of native and glycated bovine serum albumin and human transferrin, and for glycation studies of these proteins.
Permanent Link: http://hdl.handle.net/11104/0216665