A New Low-Cost Procedure for Detecting Nucleic Acids in Low-Incidence Samples: A Case Study of Detecting Spores of Paenibacillus larvae from Bee Debris

0384289 - ÚVGZ 2013 RIV US eng J - Journal Article
Ryba, Š. - Kindlmann, Pavel - Titěra, D. - Haklová, M. - Stopka, P.
A New Low-Cost Procedure for Detecting Nucleic Acids in Low-Incidence Samples: A Case Study of Detecting Spores of Paenibacillus larvae from Bee Debris.
Journal of Economic Entomology. Roč. 105, č. 5 (2012), s. 1487-1491. ISSN 0022-0493. E-ISSN 1938-291X
Institutional research plan: CEZ:AV0Z60870520
Keywords : polymerase chain reaction * matrix * honey bee * Apis mellifera * AFB
Subject RIV: EH - Ecology, Behaviour
Impact factor: 1.600, year: 2012
http://dx.doi.org/10.1603/EC12010

American foulbrood, because of its virulence and worldwide spread, is currently one of the most dangerous diseases of honey bees. Quick diagnosis of this disease is therefore vitally important. For its successful eradication, however, all the hives in the region must be tested. This is time consuming and costly. Therefore, a fast and sensitive method of detecting American foulbrood is needed. Here we present a method that signiÞcantly reduces the number of tests needed by combining batches of samples from different hives. The results of this method were veriÞed by testing each sample. A simulation study was used to compare the efÞciency of the new method with testing all the samples and to develop a decision tool for determining when best to use the new method. The method is suitable for testing large numbers of samples (over 100) when the incidence of the disease is low (10% or less).
Permanent Link: http://hdl.handle.net/11104/0213988