Application of laser-induced breakdown spectroscopy to the analysis of algal biomass for industrial biotechnology

0384149 - ÚPT 2013 RIV NL eng J - Journal Article
Pořízka, P. - Procházka, D. - Pilát, Zdeněk - Krajčarová, L. - Kaiser, J. - Malina, R. - Novotný, J. - Zemánek, Pavel - Ježek, Jan - Šerý, Mojmír - Bernatová, Silvie - Krzyžánek, Vladislav - Hrubanová, Kamila - Novotný, K. - Trtílek, M. - Samek, Ota
Application of laser-induced breakdown spectroscopy to the analysis of algal biomass for industrial biotechnology.
Spectrochimica Acta Part B: Atomic Spectroscopy. 74-75, AUG-SEP (2012), s. 169-176. ISSN 0584-8547. E-ISSN 1873-3565
R&D Projects: GA ČR GAP205/11/1687; GA MŠMT ED0017/01/01
Institutional support: RVO:68081731
Keywords : LIBS * double-pulse * water-jet * algal biomass * biotechnology
Subject RIV: BH - Optics, Masers, Lasers
Impact factor: 3.141, year: 2012

We report on the application of laser-induced breakdown spectroscopy (LIBS) to the determination of elements distinctive in terms of their biological significance (such as potassium, magnesium, calcium, and sodium) and to the monitoring of accumulation of potentially toxic heavy metal ions in living microorganisms (algae); in order to trace e.g. the influence of environmental exposure and other cultivation and biological factors having an impact on them. Algae cells were suspended in liquid media or piesented in a form of adherent cell mass on a surface (biofilm) and, consequently, characterized using their spectra. In our feasibility study we used three different experimental arrangements employing double-pulse LIBS technique in order to improve on analytical selectivity and sensitivity for potential industrial biotechnology applications, e.g. for monitoring of mass production of commercial biofuels, utilization in the food industry and control of the removal of heavy metal ions from industrial waste waters.
Permanent Link: http://hdl.handle.net/11104/0213881