Next-Generation Sequencing Reveals the Impact of Repetitive DNA Across Phylogenetically Closely Related Genomes of Orobanchaceae

0381629 - BC 2013 RIV US eng J - Journal Article
Piednoël, M. - Aberer, A.J. - Schneeweiss, G. M. - Macas, Jiří - Novák, Petr - Gundlach, H. - Temsch, E.M. - Renner, S.S.
Next-Generation Sequencing Reveals the Impact of Repetitive DNA Across Phylogenetically Closely Related Genomes of Orobanchaceae.
Molecular Biology and Evolution. Roč. 29, č. 11 (2012), s. 3601-3611. ISSN 0737-4038. E-ISSN 1537-1719
Institutional research plan: CEZ:AV0Z50510513
Institutional support: RVO:60077344
Keywords : next-generation sequencing * polyploidy * genome size * Ty3/Gypsy * transposable elements
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 10.353, year: 2012

We used next-generation sequencing to characterize the genomes of nine species of Orobanchaceae of known phylogenetic relationships, different life forms, and including a polyploid species. The study species are the autotrophic, nonparasitic Lindenbergia philippensis, the hemiparasitic Schwalbea americana, and seven nonphotosynthetic parasitic species of Orobanche (Orobanche crenata, Orobanche cumana, Orobanche gracilis (tetraploid), and Orobanche pancicii) and Phelipanche (Phelipanche lavandulacea, Phelipanche purpurea, and Phelipanche ramosa). Ty3/Gypsy elements comprise 1.93%-28.34% of the nine genomes and Ty1/Copia elements comprise 8.09%-22.83%. When compared with L. philippensis and S. americana, the nonphotosynthetic species contain higher proportions of repetitive DNA sequences, perhaps reflecting relaxed selection on genome size in parasitic organisms.
Permanent Link: http://hdl.handle.net/11104/0212054