Enhancement of immune response towards non-lipidized Borrelia burgdorferi recombinant OspC antigen by binding onto the surface of metallochelating nanoliposomes with entrapped lipophilic derivatives of norAbuMDP

0379796 - ÚOCHB 2013 RIV NL eng J - Journal Article
Křupka, M. - Mašek, J. - Bartheldyová, E. - Turánek Knotigová, P. - Plocková, J. - Korvasová, Z. - Škrabalová, M. - Koudelka, Š. - Kulich, P. - Zachová, K. - Czerneková, L. - Strouhal, O. - Horynová, M. - Šebela, M. - Miller, A. D. - Ledvina, Miroslav - Raška, M. - Turánek, J.
Enhancement of immune response towards non-lipidized Borrelia burgdorferi recombinant OspC antigen by binding onto the surface of metallochelating nanoliposomes with entrapped lipophilic derivatives of norAbuMDP.
Journal of Controlled Release. Roč. 160, č. 2 (2012), s. 374-381. ISSN 0168-3659. E-ISSN 1873-4995
R&D Projects: GA ČR(CZ) GAP304/10/1951; GA TA ČR TA01011165; GA AV ČR(CZ) KAN200520703; GA AV ČR KAN200100801
Grant - others:GA UP(CZ) LF-2010-014; GA UP(CZ) LF-2011-002
Institutional research plan: CEZ:AV0Z40550506
Keywords : Borrelia burgdorferi * OspC * metallochelating liposomes * MDP
Subject RIV: CC - Organic Chemistry
Impact factor: 7.633, year: 2012

Lyme disease caused by spirochete Borrelia burgdorferi sensu lato, is a tick-born illness. If the infection is not eliminated by the host immune system and/or antibiotics, it may further disseminate and cause severe chronic complications. The immune response to Borrelia is mediated by phagocytic cells and by Borrelia-specific complement-activating antibodies associated with Th1 cell activation. A new experimental vaccine was constructed using non-lipidized form of recombinant B. burgdorferi s.s. OspC protein was anchored by metallochelating bond onto the surface of nanoliposomes containing novel nonpyrogenic normuramyglycopeptides modified at carboxy terminus of the peptide part of their molecules by bulky lipophilic acyl residue. After i.d. immunization, the experimental vaccines surpassed Alum with respect to OspC-specific titers of IgG2a, IgG2b isotypes when analog modified with branch fatty acid was used and IgG3, IgM isotypes when analog modified with linear fatty acid was used. Both adjuvants exerted a high adjuvant effect comparable or better than MDP and proved themselves as nonpyrogenic.
Permanent Link: http://hdl.handle.net/11104/0210671