Polymorphisms in miRNA binding sites of nucleotide excision repair genes and colorectal cancer risk

0379749 - ÚEM 2013 RIV GB eng J - Journal Article
Naccarati, Alessio - Pardini, Barbara - Landi, S. - Landi, D. - Slyšková, Jana - Novotný, J. - Levý, M. - Poláková, Veronika - Lipská, L. - Vodička, Pavel
Polymorphisms in miRNA binding sites of nucleotide excision repair genes and colorectal cancer risk.
Carcinogenesis. Roč. 33, č. 7 (2012), s. 1346-1351. ISSN 0143-3334. E-ISSN 1460-2180
R&D Projects: GA ČR GAP304/10/1286; GA ČR GP305/09/P194
Institutional research plan: CEZ:AV0Z50390703
Keywords : DNA repair * polymorphisms * miRNA binding sites
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 5.635, year: 2012

Regulation of and coordination between DNA repair genes is fundamental for maintaining genome stability, and post-transcriptional gene regulation by microRNAs is of particular relevance. In this context, the presence of single nucleotide polymorphisms (SNPs) within the 3´-untranslated regions of target DNA repair genes could alter the binding with specific miRNAs,modulating gene expression and affecting cancer susceptibility. In this study, we investigated the role of genetic variations in miRNA-binding sites of nucleotide excision repair (NER) genes in association with colorectal cancer (CRC) risk. We tested those SNPs based on 28 NER genes in 1098 colorectal cancer cases and 1469 healthy controls from the Czech Republic. Rs7356 in RPA2 and rs4596 in GTF2H1 were associated with colorectal cancer risk. After stratification for tumor location, the association of both SNPs was significant only for rectal cancer (rs7356: OR 1.52, 95% CI 1.02–2.26, P = 0.04 and rs4596: OR 0.69, 95% CI 0.50–0.94, P = 0.02; results not adjusted for multiple testing). Variation in miRNA target binding sites in the 3´-untranslated region of NER genes may be important for modulating CRC risk, with a different relevance according to tumor location.
Permanent Link: http://hdl.handle.net/11104/0210633