Microradiagraphy of biological samples with Timepix

0379076 - BC 2013 RIV GB eng J - Journal Article
Dammer, J. - Weyda, František - Beneš, J. - Sopko, V. - Jakůbek, J. - Vondráček, V.
Microradiagraphy of biological samples with Timepix.
Journal of Instrumentation. Roč. 6, C11005 (2011), s. 1-6. ISSN 1748-0221. E-ISSN 1748-0221.
[International workshop on radiation imaging detectors /13./. Zurich, 03.07.2011-07.07.2011]
R&D Projects: GA MŠMT 2B06005
Grant - others:GA MŠk(CZ) LC06041; GA AV ČR(CZ) IAA600550614; GA MŠk(CZ) 2B06007; Research Program(CZ) 6840770029; Research Program(CZ) 6840770040; GA MŠk-spolupráce s CERN(CZ) 1P04LA211
Program: LC; IA; 2B; 1P
Institutional research plan: CEZ:AV0Z50070508
Keywords : X-ray detectors * X-ray radiography and digital radiography (DR) * pixelated detectors and associated VLSI electronics
Subject RIV: EA - Cell Biology
Impact factor: 1.869, year: 2011
http://iopscience.iop.org/1748-0221/6/11/C11005/pdf/1748-0221_6_11_C11005.pdf

Microradiography is an imaging technique using X-rays in the study of internal structures of objects. This rapid and convenient imaging tool is based on differential X-ray attenuation by various tissues and structures within the biological sample. The non-absorbed radiation is detected with a suitable detector and creates a radiographic image. In order to detect the differential properties of X-rays passing through structures sample with different compositions, an adequate high-quality imaging detector is needed. We describe the recently developed radiographic apparatus, equipped with Timepix semiconductor pixel detector. The detector is used as an imager that counts individual photons of ionizing radiation, emitted by an X-ray tube FeinFocus with tungsten, copper or molybdenum anode. Thanks to the wide dynamic range, time over threshold mode-counter is used as Wilkinson type ADC allowing direct energy measurement in each pixel of Timepix detector and its high spatial resolution better than 1 m m, the setup is particularly suitable for radiographic imaging of small biological samples. We are able to visualize some internal biological processes and also to resolve the details of insects (morphology) using different anodes. These anodes generate different energy spectra. These spectra depend on the anode material. The resulting radiographic images varies according to the selected anode. Tiny live insects are an ideal object for our studies.
Permanent Link: http://hdl.handle.net/11104/0210354