Light scattering evidence of selective protein fouling on biocompatible block copolymer micelles

0378727 - ÚMCH 2013 RIV GB eng J - Journal Article
Giacomelli, F. C. - Štěpánek, Petr - Schmidt, V. - Jäger, Eliezer - Jäger, Alessandro - Giacomelli, C.
Light scattering evidence of selective protein fouling on biocompatible block copolymer micelles.
Nanoscale. Roč. 4, č. 15 (2012), s. 4504-4514. ISSN 2040-3364. E-ISSN 2040-3372
R&D Projects: GA ČR GAP208/10/1600
Institutional research plan: CEZ:AV0Z40500505
Keywords : copolymer micelles * protein fouling * light scattering
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 6.233, year: 2012

Selective protein fouling on block copolymer micelles with well-known potential for tumour-targeting drug delivery was evidenced by using dynamic light scattering measurements. The stability and interaction of block copolymer micelles with model proteins (BSA, IgG, lysozyme and CytC) is reported for systems featuring a hydrophobic (poly[2-(diisopropylamino)-ethyl methacrylate]) (PDPA) core and hydrophilic coronas comprising poly(ethylene oxide)/poly(glycerol monomethacrylate) (PEOb-PG2MA) or poly[2-(methacryloyloxy)ethyl phosphorylcholine] (PMPC). The results revealed that protein size and hydrophilic chain density play important roles in the observed interactions. The successful protein-repellent characteristic of these nanoparticles is attributed to a high hydrophilic surface chain density (>0.1 chains per nm2) and to the length of the hydrophilic chains.
Permanent Link: http://hdl.handle.net/11104/0210129