Survey of several methods deproteinizing human plasma before and within the chloroformate-mediated treatment of amino/carboxylic acids quantitated by gas chromatography

0377579 - BC 2013 RIV NL eng J - Journal Article
Hušek, Petr - Švagera, Z. - Hanzlíková, D. - Šimek, Petr
Survey of several methods deproteinizing human plasma before and within the chloroformate-mediated treatment of amino/carboxylic acids quantitated by gas chromatography.
Journal of Pharmaceutical and Biomedical Analysis. 67-68, AUG-SEP (2012), s. 159-162. ISSN 0731-7085. E-ISSN 1873-264X
R&D Projects: GA MZd NS9755; GA ČR GAP206/10/2401
Institutional research plan: CEZ:AV0Z50070508
Keywords : plasma (lipo)protein precipitation * chloroformate-treated supernatant * S-amino acids
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 2.947, year: 2012
http://www.sciencedirect.com/science/article/pii/S0731708512002245

Trichloroacetic acid, perchloric acid, phosphotungstic acid, acetonitrile, methanol and some other organic solvents were evaluated for their ability to provide protein and lipid-free plasma supernatants. The residual proteins, total cholesterol and triacylglycerols were assayed in the supernatant on a Beckman Analyzer. The free cholesterol and the neutral lipids were further analyzed by means of high-temperature GC analysis. The conditions for the deproteinizing step were optimized for minimal lipoprotein disruption. Three plasma sulfur and aromatic amino acids were used as model compounds to evaluate compatibility of the precipitation methods with a subsequent methyl chloroformate-mediated derivatization and GC–MS analysis. The results of the total homocysteine assay matched well with that obtained using a commercial immunoassay. Precipitation with trichloroacetic acid has proven to be a method of choice for the analysis of the acido-basic analytes by GC–MS via chloroformates.
Permanent Link: http://hdl.handle.net/11104/0209700