Differential response of Drosophila cell lines to extracellular adenosine

0376113 - BC 2013 RIV GB eng J - Journal Article
Fleischmannová, J. - Kučerová, Lucie - Šandová, Kateřina - Steinbauerová, Veronika - Brož, Václav - Šimek, Petr - Žurovec, Michal
Differential response of Drosophila cell lines to extracellular adenosine.
Insect Biochemistry and Molecular Biology. Roč. 42, č. 5 (2012), s. 321-331. ISSN 0965-1748. E-ISSN 1879-0240
R&D Projects: GA MŠMT(CZ) LC06077
Grant - others:AV ČR(CZ) KJB501410801; European Community´s Seventh Framwork Programme (FP7/2007-2013)(CZ) 229518
Institutional research plan: CEZ:AV0Z50070508
Institutional support: RVO:60077344
Keywords : adenosine recycling * nucleoside transport * Mbn2
Subject RIV: CE - Biochemistry
Impact factor: 3.234, year: 2012
http://www.sciencedirect.com/science/article/pii/S0965174812000033

Adenosine (Ado) is a crucial metabolite that influences proliferation of several vertebrate and invertebrate cells. Key proteins regulating Ado signaling, transport and metabolism are conserved among vertebrates and invertebrates. Here we show that Ado negatively influences viability, changes morphology and mitochondrial polarity of the Drosophila imaginal disc cell line (Cl.8ţ) via a mechanism exclusively dependent on cellular Ado uptake. High transport of Ado is followed by ATP production as a part of Ado salvation, which at higher concentrations may interfere with cellular homeostasis. In contrast, hematopoietic cell line Mbn2, which grows well in high Ado concentration, preferentially uses adenosine deaminase as a part of the purine catabolic pathway. Our results show that different types of Drosophila cell lines use different pathways for Ado conversion and suggest that such differences may be an important part of complex mechanisms maintaining energy homeostasis in the body.
Permanent Link: http://hdl.handle.net/11104/0208606