The strength of integrin binding between neutrophils and endothelial cells

0375893 - ÚH 2013 RIV DE eng J - Journal Article
Labrador, V. - Říha, Pavel - Muller, S. - Dumas, D. - Wang, X. - Stoltz, J. F.
The strength of integrin binding between neutrophils and endothelial cells.
European Biophysics Journal With Biophysics Letters. Roč. 32, č. 8 (2003), s. 684-688. ISSN 0175-7571. E-ISSN 1432-1017
R&D Projects: GA ČR GA305/01/1605
Institutional research plan: CEZ:AV0Z2060917
Keywords : endothelium * integrins * neutrophil adhesion * scanning microscopy
Subject RIV: BO - Biophysics
Impact factor: 1.769, year: 2003

The firm adhesion of activated polymorphonuclear neutrophils to endothelial cells in blood vessels is achieved through binding of the integrin intercellular adhesion molecule. To contribute to the better understanding of this adhesion step, our investigation is aimed at the relationship between integrin expression and the strength of neutrophil binding to endothelial cells. Flow cytometry and 3D scanning microscopy are used to study integrin expression and distribution, respectively. It is found that CD11b/CD18 integrin expression is localized in clusters distributed irregularly over the neutrophil surface. After cell activation, the cluster distribution polarizes, increasing the local CD11b/CD18 density concurrently with nearly doubled integrin expression. The neutrophil adhesion efficiency is measured in a flow chamber coated successively by various substrates, including endothelial cells in an activated state. Analysis of the flow dependence of the number of attached cells reveals the prevailing number of neutrophils with stronger binding to the endothelium when both cells are in the activated state in comparison with non-activated cells.
Permanent Link: http://hdl.handle.net/11104/0208435