Silica-coated La.sub.0.75./sub.Sr.sub.0.25./sub.MnO.sub.3./sub. nanoparticles for magnetically driven DNA isolation

0375107 - FZÚ 2012 RIV DE eng J - Journal Article
Trachtová, Š. - Kaman, Ondřej - Španová, A. - Veverka, Pavel - Pollert, Emil - Rittich, B.
Silica-coated La_0.75Sr_0.25MnO₃ nanoparticles for magnetically driven DNA isolation.
Journal of Separation Science. Roč. 34, č. 21 (2011), s. 3077-3082. ISSN 1615-9306. E-ISSN 1615-9314
R&D Projects: GA AV ČR KAN200200651
Institutional research plan: CEZ:AV0Z10100521
Keywords : DNA isolation * lanthanum manganese perovskite * nanoparticles
Subject RIV: BM - Solid Matter Physics ; Magnetism
Impact factor: 2.733, year: 2011

Magnetic La0.75Sr0.25MnO3 nanoparticles possessing an approximately 20-nm-thick silica shell (LSMO(0.25)@SiO2) were characterised and tested for the isolation of PCR-ready bacterial DNA. The results presented here show that the nanoparticles do not interfere in PCR. DNA was apparently reversibly adsorbed on their silica shell from the aqueous phase system (16% PEG 6000–2M NaCl). The method proposed was used for DNA isolation from complex food samples (dairy products and probiotic food supplements). The isolated DNA was compatible with PCR. The main advantages of the nanoparticles tested for routine use were their high colloidal stability allowing a more precise dosage and therefore high reproducibility of DNA isolation.
Permanent Link: http://hdl.handle.net/11104/0207855