3D visualization of collagen in artificial scaffolds using two-photon excitation and second-harmonic generation imaging

0373200 - FGÚ 2012 RIV IT eng O - Others
Čapek, M. - Burdíková, Zuzana - Filová, Eva - Košťáková, E. - Janáček, Jiří
3D visualization of collagen in artificial scaffolds using two-photon excitation and second-harmonic generation imaging.
Proceedings 10th Multinational Congress on Microscopy 2011. Urbino: Societa Italiana Scienze Micriscopiche, 2011. s. 261-262
R&D Projects: GA MŠMT(CZ) LC06063; GA MŠMT(CZ) MEB0810092; GA ČR GAP501/10/0340; GA ČR(CZ) GA304/09/0733; GA AV ČR IAA500390702; GA MŠMT 7E09088
Grant - others:GA MŠk(CZ) ME10145
Institutional research plan: CEZ:AV0Z50110509; CEZ:AV0Z50390512
Keywords : multi-photon microscopy * artificial scaffolds * collagen
Subject RIV: EA - Cell Biology

Nonlinear microscopy can employ two different types of signals, fluorescence and second-harmonic generation (SHG), to image biological structures with subcellular resolution. SHG imaging was used for the identification of type II collagen in an extracellular matrix. Chondrocyte proliferation was linked to production of the extracellular matrix proteins. Collagen as a typical extracellular cartilage protein was visualized, first, by CLSM using fluorescently labeled monoclonal antibodies against type II collagen. Conventional confocal microscopy images were compared with the SHG imaging in both forward and backward nondescanned modes. Well detectable signals were observed in PCL and gelatine scaffolds. Detection of collagen by SHG imaging corresponded well with collagen detection by immunohistochemical staining
Permanent Link: http://hdl.handle.net/11104/0206340