Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization

0371924 - BTÚ 2012 RIV NO eng J - Journal Article
Brevik, A. - Rusňáková, Vendula - Duale, N. - Slagsvold, H.H. - Olsen, A.-K. - Storeng, R. - Kubista, Mikael - Brunborg, G. - Lindeman, B.
Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization.
Reproductive Toxicology. Roč. 32, č. 4 (2011), s. 463-471. ISSN 0890-6238. E-ISSN 1873-1708
R&D Projects: GA AV ČR(CZ) IAA500520809
Institutional research plan: CEZ:AV0Z50520701
Keywords : Single-cell gene expression * Glycidamide * Acrylamide
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.226, year: 2011

Recognition of early determinants of disease onset has sparked an interest in paternally transmitted factors and their impact on the developing embryo. Acrylamide (AA),a widely distributed xenobiotic compound,is converted to its active metabolite glycidamide (GA) by the CYP2E1 enzyme.Based on its capacity to induce dominant lethal mutations,we hypothesized that paternal GA exposure would have a negative impact on embryonic genome activation,via GA-DNA and protamine adducts persisting in the fertilizing sperm. Using a combination of in vitro fertilization (IVF) techniques and RT-qPCR single embryo gene expression (SEGE), we studied the expression of key DNA repair genes and genes important for embryo development, at the 1-, 2-, 4- and 8-cell stage of the developing mouse embryo. Compared to controls paternal GA-exposure gave rise to an altered pattern of embryonic gene expression, with an initial reduced expression at early stages followed by increased expression at the 8-cell stage.
Permanent Link: http://hdl.handle.net/11104/0205334