Number of the records: 1
Efficient biolistic transformation of the moss Physcomitrella patens
- 1.0370792 - ÚEB 2012 RIV NL eng J - Journal Article
Šmídková, Markéta - Holá, M. - Angelis, Karel
Efficient biolistic transformation of the moss Physcomitrella patens.
Biologia Plantarum. Roč. 54, č. 4 (2010), s. 777-780. ISSN 0006-3134. E-ISSN 1573-8264
R&D Projects: GA ČR GA521/04/0971; GA AV ČR IBS5038304; GA MŠMT 1M0505; GA MŠMT(CZ) LC06004
Institutional research plan: CEZ:AV0Z50380511
Keywords : Helios biolistic gun * moss protonemal tissue * particle size
Subject RIV: EI - Biotechnology ; Bionics
Impact factor: 1.582, year: 2010
High rates of homologous recombination (HR) in comparison to other plants make the moss Physcomitrella patens an attractive model organism for genetic studies as well as biotechnological applications. We describe a simple protocol for the efficient biolistic transformation of protonemal tissue with minimum tissue handling steps. The transformation efficiency depends on the biolistic conditions. The bombardment of tissue with 1 mu m gold particles yielded between 20 and 40 stable transformants per 1 mu g of DNA. Transformation with circular plasmids generates higher frequencies of random transgene integration, whereas linear plasmids are more efficient in generating gene-targeted insertions.
Permanent Link: http://hdl.handle.net/11104/0204485
Number of the records: 1