Wound healing gene therapy: cartilage regeneration induced by vascular endothelial growth factor plasmid

0370725 - ÚMG 2012 RIV US eng J - Journal Article
Kološtová, K. - Taltynov, O. - Pintérová, D. - Boubelík, M. - Raška, O. - Hozák, Pavel - Jirkovská, M. - Bobek, V.
Wound healing gene therapy: cartilage regeneration induced by vascular endothelial growth factor plasmid.
American Journal of Otolaryngology. Roč. 33, č. 1 (2012), s. 68-74. ISSN 0196-0709. E-ISSN 1532-818X
Institutional research plan: CEZ:AV0Z50520514
Keywords : BALB/c mouse strain * significant angiogenesis * cartilage repair * phVEGF(165) injection
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.228, year: 2012

Vascular endothelial growth factor (VEGF) is the most critical regulator of vascular formation. Using plasmid transfer of human complementary DNA VEGF165 (phVEGF165) we performed therapeutic angiogenesis in experimental skin and cartilage trauma in BALB/c mice. At 6 weeks of age all mice were ear-punched and phVEGF165 injected into the first ear and vector without insert or saline injection into the second one. The healing process was followed. The hollow diameter was measured and histological sections of experimental and control pinnae were taken regularly after experimental injury for hematoxylin and eosin and periodic acid Schiff staining and for human VEGF immunocytochemistry. Human VEGF expression was also checked by real-time PCR in formalin-fixed, paraffin-embedded tissue sections. Significant angiogenesis promotion and cartilage repair were observed in the mice. Administering phVEGF165 thus leads to faster cartilage regeneration even if not only on the angiogenic basis.
Permanent Link: http://hdl.handle.net/11104/0204436