Analysis of two abundant, highly related satellites in the allotetraploid Nicotiana arentsii using double-strand conformation polymorphism analysis and sequencing

0370431 - BFÚ 2012 RIV US eng J - Journal Article
Matyášek, Roman - Fulneček, Jaroslav - Leitch, A.R. - Kovařík, Aleš
Analysis of two abundant, highly related satellites in the allotetraploid Nicotiana arentsii using double-strand conformation polymorphism analysis and sequencing.
New Phytologist. Roč. 192, č. 3 (2011), s. 747-759. ISSN 0028-646X. E-ISSN 1469-8137
R&D Projects: GA ČR(CZ) GA206/09/1751; GA ČR(CZ) GAP501/10/0208; GA MŠMT(CZ) LC06004
Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Keywords : DNA-curvature * subtelomeric satellite repeats * allopolyploidy
Subject RIV: BO - Biophysics
Impact factor: 6.645, year: 2011

In allotetraploid Nicotiana arentsii, we examined the fate of homologous subtelomeric satellites NUNSSP and NWISSP inherited from diploid progenitors N. undulata and N. wigandioides, respectively. While the NUNSSP monomers were highly homogeneous in nucleotide sequences, the NWISSP monomers formed two separate clades. Likewise, the genomic NUNSSP monomers showed less DNA conformation heterogeneity than NWISSP monomers, with distinct conformations. Sequence, structural and chromosomal features of the parental satellites were faithfully inherited by N. arentsii. Our study revealed that inter-genomic homogenisation of subtelomeric satellite repeats does not occur in N. arentsii allotetraploid. We propose that the sequence and structural divergence of subtelomeric satellites may render allopolyploid chromosomes less vulnerable to intergenomic exchanges.
Permanent Link: http://hdl.handle.net/11104/0204234