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Sample drift correction in 3D fluorescence photoactivation localization microscopy
- 1.0370168 - FGÚ 2012 RIV US eng J - Journal Article
Mlodzianoski, M. J. - Schreiner, J. M. - Callahan, S. P. - Smolková, Katarína - Dlasková, Andrea - Šantorová, Jitka - Ježek, Petr - Bewersdorf, J.
Sample drift correction in 3D fluorescence photoactivation localization microscopy.
Optics Express. Roč. 19, č. 16 (2011), s. 15009-15019. ISSN 1094-4087
R&D Projects: GA ČR GAP302/10/0346; GA MŠMT(CZ) ME09029; GA AV ČR(CZ) KJB500110902
Institutional research plan: CEZ:AV0Z50110509
Keywords : Biplane FPALM microscopy * mitochondria * sample drift correction
Subject RIV: ED - Physiology
Impact factor: 3.587, year: 2011
The recent development of diffraction-unlimited far-field fluorescence microscopy has overcome the classical resolution limit of similar to 250 nm of conventional light microscopy by about a factor of ten. The improved resolution, however, is susceptible to sample drift on a much finer scale than previously relevant. Here we discuss an approach that corrects for three-dimensional (3D) drift in images of fixed samples without the requirement for fiduciary markers or instrument modification. By imaging mitochondria with Biplane FPALM we show our algorithm’s feasibility in a practical application
Permanent Link: http://hdl.handle.net/11104/0204047
Number of the records: 1