Regulation of Src family kinases involved in T cell receptor signaling by protein-tyrosine phosphatase CD148

0370118 - ÚMG 2012 RIV US eng J - Journal Article
Štěpánek, Ondřej - Kalina, T. - Dráber, Peter - Skopcová, Tereza - Svojgr, K. - Angelisová, Pavla - Hořejší, Václav - Weiss, A. - Brdička, Tomáš
Regulation of Src family kinases involved in T cell receptor signaling by protein-tyrosine phosphatase CD148.
Journal of Biological Chemistry. Roč. 286, č. 25 (2011), s. 22101-22112. ISSN 0021-9258. E-ISSN 1083-351X
R&D Projects: GA MŠMT 2B06064; GA MŠMT 1M0506
Institutional research plan: CEZ:AV0Z50520514
Keywords : CD148 * tyrosine phosphatase * Src family kinases
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.773, year: 2011

CD148 is a receptor-like protein-tyrosine phosphatase inhibiting transduction of signals in non-hematopoietic cells. We describe striking differences in CD148 expression between human and murine thymocyte subsets, the only unifying feature being the absence of CD148 during the positive selection when the major developmental block occurs under CD45 deficiency. CD148 has both activating and inhibitory effects on the SFKs involved in TCR signaling. In the absence of CD45, activating effects prevail, resulting in functional complementation of CD45 deficiency in human T cell lines. This is independent of the tyrosines in the CD148 C-terminal tail, contradicting the recently proposed phosphotyrosine displacement model as a mechanism of SFK activation by CD148. Differential effects of CD148 in T cells and other leukocyte subsets cannot be explained by the CD148 inability to activate T cell SFKs but rather by its dual inhibitory/activatory function and specific expression pattern.
Permanent Link: http://hdl.handle.net/11104/0204013