Cyclosporine A-loaded and stem cell-seeded electrospun nanofibers for cell-based therapy and local immunosuppression

0370057 - ÚMG 2012 RIV NL eng J - Journal Article
Holáň, Vladimír - Chudíčková, Milada - Trošan, Peter - Svobodová, Eliška - Krulová, Magdalena - Kubinová, Šárka - Syková, Eva - Širc, Jakub - Michálek, Jiří - Juklíčková, M. - Munzarová, M. - Zajícová, Alena
Cyclosporine A-loaded and stem cell-seeded electrospun nanofibers for cell-based therapy and local immunosuppression.
Journal of Controlled Release. Roč. 156, č. 3 (2011), s. 406-412. ISSN 0168-3659. E-ISSN 1873-4995
R&D Projects: GA AV ČR KAN200520804; GA ČR GAP304/11/0653; GA ČR(CZ) GAP301/11/1568; GA ČR GD310/08/H077; GA MŠMT 1M0506
Institutional research plan: CEZ:AV0Z50390512; CEZ:AV0Z40500505; CEZ:AV0Z50520514
Keywords : nanofibers * immunosuppression * cell transfer
Subject RIV: EC - Immunology
Impact factor: 5.732, year: 2011

The authors described preparation and construction of a novel form of nanofiber scaffold which is loaded with immunosuppressive drug cyclosporin A (CsA). The incorporation of CsA did not influence the diameter, shape, porosity or uniformity of the nanofibers. This scaffold has an optimal kinetic of drug release and can serve for a local immunosuppression of immune reactions. The covering of skin allografts with CsA-loaded nanofibers significantly attenuated the expression of the genes for the proinflammatory cytokines. The CsA-containing nanofibers can be seeded with stem cells and thus can be used for cell transfer in regenerative medicine. The results suggest that CsA-loaded electrospun nanofibers prepared from the biodegradable polymer poly(L-lactic acid) can serve as effective drug carriers for the local/topical suppression of an inflammatory reaction and simultaneously as scaffolds for cell transfer for tissue repair and regeneration.
Permanent Link: http://hdl.handle.net/11104/0203969