Number of the records: 1
Evaluation of basic mitochondrial functions using rat tissue homogenates
- 1.0369964 - FGÚ 2012 RIV NL eng J - Journal Article
Pecinová, Alena - Drahota, Zdeněk - Nůsková, Hana - Pecina, Petr - Houštěk, Josef
Evaluation of basic mitochondrial functions using rat tissue homogenates.
Mitochondrion. Roč. 11, č. 5 (2011), s. 722-728. ISSN 1567-7249. E-ISSN 1872-8278
R&D Projects: GA MZd(CZ) NS9759; GA ČR(CZ) GAP303/11/0970; GA MŠMT(CZ) 1M0520; GA MŠMT OC08017
Institutional research plan: CEZ:AV0Z50110509
Keywords : oxidative phosphorylation * isolated mitochondria * tissue homogenates * respiratory control * membrane potential
Subject RIV: FB - Endocrinology, Diabetology, Metabolism, Nutrition
Impact factor: 3.615, year: 2011
The analysis of rat heart, liver, brain and kidney by oxygraphy, enzyme activities, membrane potential and BN/SDS-PAGE Western blotting demonstrated that tissue homogenates can fully substitute for isolated mitochondria, providing comparable qualitative mitochondrial parameters. The use of homogenate avoids the loss of the majority of mitochondria during their isolation. Only 50–100 mg of the tissue are required for the complex OXPHOS analysis
Permanent Link: http://hdl.handle.net/11104/0203900
Number of the records: 1