Amino Acid Analysis. Methods and Protocols

0369909 - BC 2012 RIV US eng M - Monography Chapter
Šimek, Petr - Hušek, Petr - Zahradníčková, Helena
Heptafluorobutyl chloroformate - based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography.
Amino Acid Analysis. Methods and Protocols. Vol. 828. New York: Springer Science & Business Media, 2012 - (Alterman, M.; Hunziker, P.), s. 137-152. ISBN 978-1-61779-444-5
R&D Projects: GA MŠMT 7F09028; GA MZd NS9755; GA ČR GA203/09/2014
Grant - others:Národní vzdělávací fond(CZ) A/CZ0046/1/0018
Institutional research plan: CEZ:AV0Z50070508
Keywords : amino acid * serum * plasma
Subject RIV: CB - Analytical Chemistry, Separation

Gas chromatography (GC) is a commonly used technique in amino acid analysis (AAA). However, one of the requirements of the application of GC for AAA is a need for the polar analytes to be converted into their volatile, thermally stable derivatives. In the last two decades, alkyl chloroformates have become attractive derivatization reagents. The reagents react immediately with most amino acid functional groups in aqueous matrices and the process can easily be coupled with liquid–liquid extraction of the resulting less-polar derivatives into immiscible organic phase. Here, we describe a simple protocol for in situ derivatization of amino acids with heptafluorobutyl chloroformate followed by subsequent chiral as well as nonchiral GC/mass spectrometric analysis on a respective nonpolar fused silica and an enantioselective Chirasil-Val capillary column.
Permanent Link: http://hdl.handle.net/11104/0203861