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Evidence for the presence of proteolytically active secreted aspartic proteinase 1 of Candida parapsilosis in the cell wall
- 1.0369816 - ÚOCHB 2012 RIV US eng J - Journal Article
Vinterová, Zuzana - Šanda, Miloslav - Dostál, Jiří - Hrušková-Heidingsfeldová, Olga - Pichová, Iva
Evidence for the presence of proteolytically active secreted aspartic proteinase 1 of Candida parapsilosis in the cell wall.
Protein Science. Roč. 20, č. 12 (2011), s. 2004-2012. ISSN 0961-8368. E-ISSN 1469-896X
R&D Projects: GA MŠMT(CZ) LC531; GA ČR GA310/09/1945
Institutional research plan: CEZ:AV0Z40550506
Keywords : Candida parapsilosis * secreted aspartic proteinases * Sapp1p * cell wall * biotin * proteolytic activity
Subject RIV: CE - Biochemistry
Impact factor: 2.798, year: 2011
We found that Sapp1p of C. parapsilosis displays enzyme activity prior to secretion, and therefore, it is probably fully folded within the upper layer of the cell wall. The positioning of cell surface-associated Sapp1p was detected by cell wall protein labeling using biotinylation agents, extraction of cell wall proteins by beta-mercaptoethanol, immunochemical detection, and mass spectrometry analysis. The retaining of Sapp1p in the cell wall is not a totally random process and pathogenic yeasts might use this cell-associated proteinase activity to enhance degradation of appropriate substrates.
Permanent Link: http://hdl.handle.net/11104/0203794
Number of the records: 1