Time-Dependent Oxidation during Nano-Assisted Laser Desorption Ionization Mass Spectrometry: A Useful Tool for Structure Determination or a Source of Possible Confusion?

0369749 - MBÚ 2012 RIV US eng J - Journal Article
Pavlásková, Kateřina - Strnadová, Marcela - Strohalm, Martin - Havlíček, Vladimír - Šulc, Miroslav - Volný, Michael
Time-Dependent Oxidation during Nano-Assisted Laser Desorption Ionization Mass Spectrometry: A Useful Tool for Structure Determination or a Source of Possible Confusion?
Analytical Chemistry. Roč. 83, č. 14 (2011), s. 5661-5665. ISSN 0003-2700. E-ISSN 1520-6882
R&D Projects: GA ČR GPP206/10/P018; GA MŠMT LC545; GA MŠMT(CZ) ME10013
Institutional research plan: CEZ:AV0Z50200510
Keywords : DOUBLE-BOND POSITION * UNSATURATED FATTY-ACIDS * ELECTROSPRAY-IONIZATION
Subject RIV: CE - Biochemistry
Impact factor: 5.856, year: 2011

This work reports on a new and extremely simple approach for determination of a double bond position by a laser desorption ionization mass spectrometry. It is solely based on the catalytic properties of nanostructured surfaces used in nanoassisted laser desorption ionization experiments. These surfaces can induce oxidation of analytes, which results in a mass shift that can be detected by mass spectrometry. If a site of unsaturation is oxidized and cleaved, the m/z difference is diagnostic of the position of a double bond. By demonstrating that the oxidation depends on the analyte surface dwell time, it was proven that it is caused by the surface activity and not by the laser desorption ionization process itself. Control matrix-assisted laser desorption/ionization (MALDI)experiment showed only a limited partial oxidation and no time dependency of the process
Permanent Link: http://hdl.handle.net/11104/0203744