Association of MiR-126 with Soluble Mesothelin-Related Peptides, a Marker for Malignant Mesothelioma

0369516 - BTÚ 2012 RIV US eng J - Journal Article
Santarelli, L. - Strafella, E. - Staffolani, S. - Amati, M. - Emanuelli, M. - Sartini, D. - Pozzi, V. - Carbonari, D. - Bracci, M. - Pignotti, E. - Mazzanti, P. - Sabbatini, A. - Ranaldi, R. - Gasparini, S. - Neužil, Jiří - Tomasetti, M.
Association of MiR-126 with Soluble Mesothelin-Related Peptides, a Marker for Malignant Mesothelioma.
PLoS ONE. Roč. 6, č. 4 (2011), e18232. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR(CZ) GA204/08/0811
Institutional research plan: CEZ:AV0Z50520701
Keywords : Malignant pleural mesothelioma * microRNAs * soluble mesothelin-related peptides
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.092, year: 2011

Improved detection methods for diagnosis of malignant mesothelioma (MM) are essential for detection and treatment. miRNAs isolated from fresh-frozen biopsies of MM patients were tested for the expression of 88 types of miRNA involved in cancerogenesis. Three significantly downregulated miRNAs were assayed in cancerous tissue and adjacent non-cancerous tissue sample pairs collected from 27 formalin-fixed, paraffin-embedded MM tissues by quantitative RT-PCR. Only miR-126 remained downregulated in the malignant tissues. The performance of the selected miR-126 as biomarker was evaluated in serum samples of asbestos-exposed subjects and MM patients and compared with controls. MiR-126 was not affected by asbestos exposure, whereas it was found associated with VEGF serum levels. Levels of miR-126 in serum, and its levels in patients' serum in association with a specific marker of MM, SMRPs, correlate with subjects at high risk to develop MM.
Permanent Link: http://hdl.handle.net/11104/0203562