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Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy
- 1.0369489 - FGÚ 2012 RIV US eng J - Journal Article
Michálek, Jan - Čapek, Martin - Kubínová, Lucie
Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy.
Microscopy Research and Technique. Roč. 74, č. 9 (2011), s. 831-838. ISSN 1059-910X. E-ISSN 1097-0029
R&D Projects: GA ČR(CZ) GA102/08/0691; GA ČR(CZ) GA304/09/0733; GA MŠMT(CZ) LC06063; GA MŠMT(CZ) ME09010
Institutional research plan: CEZ:AV0Z50110509
Keywords : confocal laser scanning microscopy * image enhancement * morphology filters
Subject RIV: JC - Computer Hardware ; Software
Impact factor: 1.792, year: 2011
In images acquired by confocal laser scanning microscopy (CLSM), regions corresponding to the same concentration of fluorophores in the specimen should be mapped to the same grayscale levels. However, in practice, due to multiple distortion effects, CLSM images of even homogeneous specimen regions suffer from irregular brightness variations, e.g., darkening of image edges and lightening of the center. We present a fast correction method based on estimating a spatially variable illumination gain, and multiplying acquired CLSM images by the inverse of the estimated gain
Permanent Link: http://hdl.handle.net/11104/0203541
Number of the records: 1