Profile distribution of humic substances molecular fractions in mountain spruce forest soil

0368843 - BC 2012 RIV SK eng J - Journal Article
Šestauberová, Martina - Novák, František
Profile distribution of humic substances molecular fractions in mountain spruce forest soil.
Phytopedon. Roč. 10, č. 1 (2011), s. 60-65. ISSN 1336-1120
Institutional research plan: CEZ:AV0Z60660521
Keywords : forest soil * fulvic acids * humic acids
Subject RIV: EH - Ecology, Behaviour

The objective of this study was to describe changes in molecular weight of humic (HA) and fulvic acids (FA) with depth of soil profile, and to state their mobility in a soil environment. Samples were isolated from individual podzol horizons of spruce forest in Trojmezí stand (Bohemian Forest, 48°46'25" N, 13°51'04" E). Their molecular weight M_r and weight-average molecular weight M_w were determined by the low pressure size exclusion chromatography. For the high-molecular HA fraction (HM-HA), M_r values over 250 kDa were found, while values of FA and the main HA fraction ranged between 6.4-7.6 kDa and 6.7-31.4 kDa, respectively. The M_r of humic substances and the proportion of HM-HA decreased with increasing soil depth. HA isolated from horizon B_hs didn't contain any high-molecular fraction and its molecular weight approached the values specified for FA.
Permanent Link: http://hdl.handle.net/11104/0203069