Genotoxicity of 7H-dibenzo[c,g]carbazole and its tissue-specific derivatives in human hepatoma HepG2 cells is related to CYP1A1/1A2 expression

0368653 - ÚEM 2012 RIV US eng J - Journal Article
Gábelová, A. - Valovičová, Z. - Mesárošová, M. - Trilecová, L. - Hrubá, E. - Marvanová, S. - Krčmář, P. - Milcová, Alena - Schmuczerová, Jana - Vondráček, J. - Machala, M. - Topinka, Jan
Genotoxicity of 7H-dibenzo[c,g]carbazole and its tissue-specific derivatives in human hepatoma HepG2 cells is related to CYP1A1/1A2 expression.
Environmental and Molecular Mutagenesis. Roč. 52, č. 8 (2011), s. 636-645. ISSN 0893-6692. E-ISSN 1098-2280
R&D Projects: GA MŠMT(CZ) 2B08005
Grant - others:GA MZE(CZ) MZE0002716202
Institutional research plan: CEZ:AV0Z50390512; CEZ:AV0Z50040702; CEZ:AV0Z50040507
Keywords : dibenzo[c,g]carbazoles * DNA strand breaks * DNA adducts
Subject RIV: DN - Health Impact of the Environment Quality
Impact factor: 3.709, year: 2011

The goal of this study was to investigate the genotoxicity of dibenzocarbazoles in human hepatoma HepG2 cells and to infer potential mechanisms underlying the biological activity of particular carcinogen. All dibenzocarbazoles, regardless the tissue specificity, induced significant DNA strand break levels and micronuclei in HepG2 cells; though a mitotic spindle dysfunction rather than a chromosome breakage was implicated in N-MeDBC-mediated micronucleus formation. Our data clearly demonstrated differences in the mechanisms involved in the biological activity of DiMeDBC and N-MeDBC in human hepatoma cells; the genotoxicity of these DBC derivatives is closely related to CYP1A1/2 expression.
Permanent Link: http://hdl.handle.net/11104/0202943