Analysis of blastomogenic activity of mammal carcinogens in Drosophila using the wts (P4) allele and RNA interference-induced P53 silencing

0368651 - BC 2012 RIV RU eng J - Journal Article
Kirsanov, K. I. - Lesovaya, E. A. - Sidorov, Roman - Belitsky, G. A. - Yakubovskaya, M. G.
Analysis of blastomogenic activity of mammal carcinogens in Drosophila using the wts (P4) allele and RNA interference-induced P53 silencing.
Russian Journal of Genetics. Roč. 47, č. 4 (2011), s. 409-416. ISSN 1022-7954. E-ISSN 1608-3369
R&D Projects: GA AV ČR IAA500070601
Grant - others:Russian Foundation for Basic Research(RU) 08-04-01596_a; Russian Foundation for Basic Research(RU) 11-04-01978_a
Institutional research plan: CEZ:AV0Z50070508
Keywords : Drosophila
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.427, year: 2011

The test for somatic mutagenesis and recombination in Drosophila is one of the widely used approaches for determination of possible carcinogenic effects of chemical compounds. The use of heterozygotes for mutant tumor suppressor gene wts enables more direct evaluation of the blastomogenic effects of chemical compounds, by tumor formation in the adult flies. This study presents evaluation of the SMART effectiveness upon the use of Drosophila heterozygotes for the wts (P4) gene, first included into the test system. The increase of the test resolution capacity compared to the literature data for the wts (P2) allele was observed. Using wts (P4) heterozygotes, a total of 20 carcinogenic compounds, and their slightly carcinogenic and non-carcinogenic analogs were tested. Specificity of the method was about 100%, and sensitivity depended on the type of the agent tested. The latter was absolute for the direct action carcinogens, with respect to carcinogens, requiring the metabolic activation.
Permanent Link: http://hdl.handle.net/11104/0202941