Phosphonate-Titanium Dioxide Assemblies: Platform for Multimodal Diagnostic-Therapeutic Nanoprobes

0368636 - ÚEM 2012 RIV US eng J - Journal Article
Řehoř, I. - Vilímová, V. - Jendelová, Pavla - Kubíček, V. - Jirák, D. - Herynek, V. - Kapcalová, Miroslava - Kotek, J. - Černý, J. - Hermann, P. - Lukeš, I.
Phosphonate-Titanium Dioxide Assemblies: Platform for Multimodal Diagnostic-Therapeutic Nanoprobes.
Journal of Medicinal Chemistry. Roč. 54, č. 14 (2011), s. 5185-5194. ISSN 0022-2623. E-ISSN 1520-4804
Grant - others:GA MŠk(CZ) OC 179; GA MŠk(CZ) COST D38
Institutional research plan: CEZ:AV0Z50390703
Keywords : MRI contrast agent * stem-cell therapy * Ti02 particles
Subject RIV: CA - Inorganic Chemistry
Impact factor: 5.248, year: 2011

Multimodal imaging-therapeutic nanoprobe TiO2@RhdGd was prepared and successfully used for in vitro and in vivo cell tracking as well as for killing of cancer cells in vitro. TiO2 nanoparticles were used as a core for phosphonic acid modified functionalities, responsible for contrast in MRI and optical imaging. The probe shows high 1H relaxivity and relaxivity density values. Presence of fluorescent dye allows for visualization by means of fluorescence microscopy. The applicability of the probe was studied, using mesenchymal stem cells, cancer HeLa cells, and T-lymphocytes. The probe did not exhibit toxicity in any of these systems. Labeled cells were successfully visualized in vitro by means of fluorescence microscopy and MRI. Furthermore, it was shown that the probe TiO2@RhdGd can be changed into a cancer cell killer upon UV light irradiation. The above stated results represent a valuable proof of a principle showing applicability of the probe design for diagnosis and therapy.
Permanent Link: http://hdl.handle.net/11104/0202928