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A non-radioactive assay for precise determination of intracellular levels of imatinib and its main metabolite in Bcr-Abl positive cells
- 1.0368600 - ÚEB 2012 RIV NL eng J - Journal Article
Mlejnek, P. - Novák, Ondřej - Doležel, P.
A non-radioactive assay for precise determination of intracellular levels of imatinib and its main metabolite in Bcr-Abl positive cells.
Talanta. Roč. 83, č. 5 (2011), s. 1466-1471. ISSN 0039-9140. E-ISSN 1873-3573
R&D Projects: GA ČR GA301/08/1649
Institutional research plan: CEZ:AV0Z50380511
Keywords : K562 cells * P-glycoprotein * Multidrug resistance * N-desmethyl imatinib * CGP 74588
Subject RIV: EF - Botanics
Impact factor: 3.794, year: 2011
Multidrug resistance (MDR) is often associated with overexpression of the P-glycoprotein (P-gp, ABCB1). It was demonstrated that the P-gp mediated efflux decreases the drug concentration in cancer cells which results in the failure of chemotherapy. However, the MDR phenotype in cancer cells obviously involves various mechanisms. Therefore, if we want to estimate a contribution of the P-gp expression to the MDR phenotype, a clear quantitative relationship between the intracellular drug level and cell sensitivity must be established. To achieve this goal, a sensitive and non-radioactive assay for precise determination of intracellular levels of imatinib and its main metabolite N-desmethyl imatinib (CGP 74588) has been developed.
Permanent Link: http://hdl.handle.net/11104/0202899
Number of the records: 1