Raman microspectroscopy based sensor of algal lipid unsaturation

0368397 - ÚPT 2012 RIV US eng C - Conference Paper (international conference)
Samek, Ota - Pilát, Zdeněk - Jonáš, Alexandr - Zemánek, Pavel - Šerý, Mojmír - Ježek, Jan - Bernatová, S. - Nedbal, L. - Trtílek, M.
Raman microspectroscopy based sensor of algal lipid unsaturation.
Optical Sensors 2011 and Photonic Crystal Fibers V (Proceedings of SPIE Vol. 8073). Bellingham: SPIE, 2011, 80730O:1-6. ISBN 978-0-8194-8663-9.
[Optical Sensors 2011 and Photonic Crystal Fibers V. Praha (CZ), 18.04.2011-20.04.2011]
R&D Projects: GA MŠMT ED0017/01/01; GA MŠMT OC08034; GA MPO FR-TI1/433
Grant - others:EC(XE) PERG 06-GA-2009-256526
Institutional research plan: CEZ:AV0Z20650511
Keywords : Raman spectroscopy * algal cells * lipids * iodine value
Subject RIV: BH - Optics, Masers, Lasers

Raman spectroscopy is a powerful tool for chemical analysis. This technique can elucidate fundamental questions about the metabolic processes and intercellular variability on a single cell level. Therefore, Raman spectroscopy can significantly contribute to the study and use of microalgae in systems biology and biofuel technology. Raman spectroscopy can be combined with optical tweezers. We have employed microfluidic system to deliver the sampled microalgae to the Raman-tweezers. This instrument is able to measure chemical composition of cells and to track metabolic processes in vivo, in real-time and label-free making it possible to detect population variability in a wide array of traits. Moreover, employing an active sorting switch, cells can be separated depending on input parameters obtained from Raman spectra. We focus on algal lipids which are promising potential products for biofuel as well as for nutrition.
Permanent Link: http://hdl.handle.net/11104/0202754