Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and In Vivo

0368327 - ÚŽFG 2012 RIV JP eng J - Journal Article
Vacková, I. - Nováková, Z. - Krylov, V. - Okada, K. - Kott, T. - Fulka, H. - Motlík, Jan
Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and In Vivo.
Journal of Reproduction and Development. Roč. 57, č. 5 (2011), s. 594-603. ISSN 0916-8818
R&D Projects: GA MZe QI101A166; GA MŠMT 1M0538
Institutional research plan: CEZ:AV0Z50450515
Keywords : Blastocyst * In vivo and in vitro * Pig * Stem cells
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.459, year: 2011

The present study was designed to extensively characterize cell lines derived from porcine blastocysts by several methodical approaches, including morphological observation, cytogenetic analysis, estimation of alkaline phosphatase activity and detection of specific marker expression at the mRNA/protein level. A comparison was made between the properties of cell lines isolated from in vivo- and in vitro-obtained blastocysts. Our results showed that 57.1% of the in vivo-obtained blastocysts attached to the feeder layer and that 33.3% of them started to grow in a monolayer. The percentage of attached in vitro-produced blastocysts was lower (24.6%), and only 6.9% of them started to grow. Outgrowths from the in vitro-produced blastocysts formed mainly trophectoderm or epithelial-like monolayer, whereas the in vivo-obtained blastocysts formed heterogeneous outgrowths that also contained cells with embryonic stem (ES)-like morphology.
Permanent Link: http://hdl.handle.net/11104/0202704