Development of ovarian hyperstimulation syndrome: interrogation of key proteins and biological processes in human follicular fluid of women undergoing in vitro fertilization

0368287 - ÚŽFG 2012 RIV GB eng J - Journal Article
Jarkovská, Karla - Skalníková, Helena - Halada, Petr - Hrabáková, Rita - Moos, J. - Rezábek, K. - Gadher, S. J. - Kovářová, Hana
Development of ovarian hyperstimulation syndrome: interrogation of key proteins and biological processes in human follicular fluid of women undergoing in vitro fertilization.
Molecular Human Reproduction. Roč. 17, č. 11 (2011), s. 679-692. ISSN 1360-9947
Institutional research plan: CEZ:AV0Z50450515; CEZ:AV0Z50200510
Keywords : biomarkers * computer modelling * human follicular fluid
Subject RIV: FK - Gynaecology, Childbirth
Impact factor: 3.852, year: 2011

Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic complication and potentially life-threatening condition resulting from excessive ovarian stimulation during assisted reproductive technologies. Our aim was to identify candidate proteins in follicular fluid (FF) using various proteomic approaches which may help to identify patients at risk of OHSS. We analysed the proteome alterations in FF from patients suffering from severe forms of OHSS (OHSS+) compared with a control group of women without or with only mild signs of OHSS (OHSS-). The 12 abundant proteins of FF were removed using an immunoaffinity system. Pools of remaining depleted proteins were applied to the two-dimensional (2D) electrophoresis and 2D liquid chromatography and proteins in differentially expressed protein spots/fractions were identified by mass spectrometry.
Permanent Link: http://hdl.handle.net/11104/0202675