Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds

0368065 - ÚFCH JH 2012 RIV GB eng J - Journal Article
Norris, S. C. P. - Humpolíčková, Jana - Amler, Evžen - Huranová, Martina - Buzgo, Matej - Macháň, Radek - Lukáš, D. - Hof, Martin
Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds.
Acta Biomaterialia. Roč. 7, č. 12 (2011), s. 4195-4203. ISSN 1742-7061. E-ISSN 1878-7568
R&D Projects: GA MŠMT(CZ) LC06063; GA AV ČR IAA500390702; GA ČR GAP304/10/1307
Institutional research plan: CEZ:AV0Z40400503; CEZ:AV0Z50390512; CEZ:AV0Z50390703; CEZ:AV0Z50520514
Keywords : fluorecence dynamics * transient binding * diffusion
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 4.865, year: 2011

Dynamic processes such as diffusion and binding/unbinding of macromolecules (e.g. growth factors or nutrients) are crucial parameters for the design and application of effective artificial tissue materials. Here, dynamics of selected macromolecules were studied in two different composite tissue engineering scaffolds containing an electrospun nanofiber mesh (polycaprolactone or hydrophobically plasma modified polyvinylalcohol–chitosan) encapsulated in agarose hydrogels by a conventional approach fluorescence recovery after photobleaching (FRAP) and a novel technique, raster image correlation spectroscopy (RICS). The two approaches are compared, and it is shown that FRAP is unable to determine processes occurring at low molecular concentrations, especially accurately separating binding/unbinding from diffusion, and its results depend on the concentration of the studied molecules.
Permanent Link: http://hdl.handle.net/11104/0006700