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Surface Plasmon Resonance Biosensor for Determination of Tetrodotoxin: Prevalidation Study
- 1.0367829 - ÚFE 2012 RIV US eng J - Journal Article
Vaisocherová, Hana - Taylor, A. D. - Jiang, S. - Hegnerová, Kateřina - Vala, Milan - Homola, Jiří - Yakes, B. - Deeds, J. - DeGrasse, S.
Surface Plasmon Resonance Biosensor for Determination of Tetrodotoxin: Prevalidation Study.
Journal of Aoac International. Roč. 94, č. 2 (2011), s. 596-604. ISSN 1060-3271. E-ISSN 1944-7922
R&D Projects: GA AV ČR KAN200670701
Institutional research plan: CEZ:AV0Z20670512
Keywords : toxin detection * biosensor * validtion
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
Impact factor: 1.199, year: 2011
A label-free surface plasmon resonance biosensor method was applied to determine tetrodotoxin (TTX) in pufferfish matrixes using an antibody inhibition assay format. A prevalidation study was conducted to demonstrate the assay performance characteristics, such as selectivity, LOD, LOQ, repeatability, reproducibility, and accuracy.The developed method was demonstrated to be capable of detecting TTX in pufferfish matrix standard samples in a broad concentration range (2-9000 ng/mL) with an LOD of 1.5 ng/mL.
Permanent Link: http://hdl.handle.net/11104/0202373
Number of the records: 1