Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment

0367748 - ÚŽFG 2012 RIV CZ eng J - Journal Article
Juhásová, Jana - Juhás, Štefan - Klíma, Jiří - Strnádel, Ján - Holubová, Monika - Motlík, Jan
Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment.
Physiological Research. Roč. 60, č. 3 (2011), s. 559-571. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA MŠMT 1M0538; GA MŠMT 2B06130
Institutional research plan: CEZ:AV0Z50450515
Keywords : miniature pig * mesenchymal stem cells * cell differentiation
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.555, year: 2011

Mesenchymal stem cells (MSCs) have been repeatedly shown to be able to repair bone defects. The aim of this study was to characterize the osteogenic differentiation of miniature pig MSCs and markers of this differentiation in vitro. Flow-cytometrically characterized MSCs were seeded on cultivation plastic (collagen I and vitronectin coated/uncoated) or plasma clot (PC)/plasma-alginate clot (PAC) scaffolds and differentiated in osteogenic medium. During three weeks of differentiation, the formation of nodules and deposition of calcium were visualized by Alizarin Red Staining. In addition, the production of alkaline phosphatase (ALP) activity was quantitatively detected by fluorescence. The expression of osteopontin, osteonectin and osteocalcin were assayed by immunohistochemistry and Western Blot analysis. We revealed a decrease of osteopontin expression in 2D and 3D environment during differentiation.
Permanent Link: http://hdl.handle.net/11104/0202308