REVIEWS IN FLUORESCENCE 2009

0367726 - ÚFCH JH 2012 RIV US eng M - Monography Chapter
Štefl, Martin - Macháň, Radek - Hof, Martin
Z-Scan Fluorescence Correlation Spectroscopy: A Powerful Tool for Determination of Lateral Diffusion in Biological Systems.
REVIEWS IN FLUORESCENCE 2009. New York: KLUWER ACADEMIC/PLENUM PUBL, 2011 - (Geddes, C.), s. 321-344. Reviews in Fluorescence, Vol. 6. ISBN 978-1-4419-9671-8
R&D Projects: GA ČR GAP208/10/1090; GA ČR GAP208/10/0376
Institutional research plan: CEZ:AV0Z40400503
Keywords : image correlation spectroscopy * single particle tracking * spectroscopy
Subject RIV: CF - Physical ; Theoretical Chemistry

The characterization of the dynamics of biological membranes is a topic which currently grasps a high level of attention. Biological membranes are extremely important as they are required for both protection and communication of eukaryotic cells. They also play a key role for transportation of nutrients into and out of the cell. Recent studies have proved that biological membranes are not homogeneous but are instead composed of microdomains, which complicate the precise determination of lateral diffusion coefficients. Z-scan fluorescence correlation spectroscopy (Z-scan FCS), one of the fluorescence fluctuation methods, is a technique which can be employed to determine lateral diffusion coefficients of membrane lipids and also membrane-associated molecules. Moreover, when Z-scan FCS is used in combination with Wawrezinieck diffusion law, lipid rafts in heterogeneous membranes can be monitored.
Permanent Link: http://hdl.handle.net/11104/0202297