Applications of phasors to in vitro time-resolved fluorescence measurements

0367710 - ÚFCH JH 2012 RIV US eng J - Journal Article
Štefl, Martin - James, N. G. - Jameson, D. M.
Applications of phasors to in vitro time-resolved fluorescence measurements.
Analytical Biochemistry. Roč. 410, č. 1 (2011), s. 62-69. ISSN 0003-2697. E-ISSN 1096-0309
R&D Projects: GA MŠMT(CZ) LC06063
Institutional research plan: CEZ:AV0Z40400503
Keywords : phasor * fraquency domain * fluorescence lifetimes
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 2.996, year: 2011

The phasor method of treating fluorescence lifetime data provides a facile and convenient approach to characterize lifetime heterogeneity and to detect the presence of excited state reactions such as solvent relaxation and Forster resonance energy transfer. The method uses a plot of M sin(Phi) versus M cos(Phi), where M is the modulation ratio and Phi is the phase angle taken from frequency domain fluorometry. A principal advantage of the phasor method is that it provides a model-less approach to time-resolved data amenable to visual inspection. Although the phasor approach has been recently applied to fluorescence lifetime imaging microscopy, it has not been used extensively for cuvette studies. In the current study, we explore the applications of the method to in vitro samples. The phasors of binary and ternary mixtures of fluorescent dyes demonstrate the utility of the method for investigating complex mixtures.
Permanent Link: http://hdl.handle.net/11104/0202289