Determination of Oxygen Radical Absorbance Capacity of Black Cumin (Nigella sativa) Seed Quinone Compounds

0367700 - ÚEB 2012 RIV US eng J - Journal Article
Tesařová, H. - Svobodová, B. - Kokoška, L. - Maršík, Petr - Přibylová, Marie - Landa, Přemysl - Vadlejch, J.
Determination of Oxygen Radical Absorbance Capacity of Black Cumin (Nigella sativa) Seed Quinone Compounds.
Natural Product Communications. Roč. 6, č. 2 (2011), s. 213-216. ISSN 1934-578X. E-ISSN 1555-9475
R&D Projects: GA ČR GA525/08/1179
Institutional research plan: CEZ:AV0Z50380511
Keywords : Antioxidative activity * Dihydrothymoquinone * Thymoquinone
Subject RIV: GM - Food Processing
Impact factor: 1.242, year: 2011
http://arl-repository.lib.cas.cz/uloziste_av/UEB-Q/cav_un_epca-0367700_01.pdf

In this study, the antioxidant capacities of main quinone constituents of Nigella saliva seeds, namely dithymoquinone (1), thymohydroquinone (2) and thymoquinone (3), were compared using DPPH and ORAC methods. The best scavenging activity was produced by 2, which showed a remarkable activity of 2.60 Trolox equivalents (TE) in a concentration range between 1.6 and 6.4 mu g/mL and IC50 value of 2.4 mu g/mL, in ORAC and DPPH assays, respectively. Contrastingly, 3 possessed only weak DPPH scavenging efficacy (IC50 = 170 mu g/mL) but significant antioxidative action of 1.91 TE in ORAC assay. No effect has been observed for 1. Additionally, modified protocol for synthesis of 2 has been developed with aim to enhance its availability for further studies as well as for its future potential use. Based on the results of this study, we conclude that 2 could be considered as a compound with prospective antioxidative properties.
Permanent Link: http://hdl.handle.net/11104/0202283