Characterization of fatty acid and triacylglycerol composition in animal fats using silver-ion and non-aqueous reversed-phase high-performance liquid chromatography/mass spectrometry and gas chromatography/flame ionization detection

0367598 - ÚOCHB 2012 RIV NL eng J - Journal Article
Lísa, M. - Netušilová, K. - Franěk, L. - Dvořáková, H. - Vrkoslav, Vladimír - Holčapek, M.
Characterization of fatty acid and triacylglycerol composition in animal fats using silver-ion and non-aqueous reversed-phase high-performance liquid chromatography/mass spectrometry and gas chromatography/flame ionization detection.
Journal of Chromatography A. Roč. 1218, č. 42 (2011), s. 7499-7510. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA ČR GA203/09/0139
Institutional research plan: CEZ:AV0Z40550506
Keywords : silver-ion HPLC * non-aqueous reversed-phase HPLC * triacylglycerol
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 4.531, year: 2011

Fatty acid (FA) and triacylglycerol (TG) composition of natural oils and fats intake in the diet has a strong influence on the human health and chronic diseases. In this work, non-aqueous reversed-phase (NARP) and silver-ion high-performance liquid chromatography with atmospheric pressure chemical ionization mass spectrometry detection and gas chromatography with flame-ionization detection (GC/FID) and mass spectrometry detection are used for the characterization of FA and TG composition in complex samples of animal fats from fallow deer, red deer, sheep, moufflon, wild boar, cock, duck and rabbit. The FA composition of samples is determined based on the GC/FID analysis of FA methyl esters. In total, 81 FAs of different acyl chain length, double bond (DB) number, branched/linear, cis-/trans- and DB positional isomers are identified. TGs in animal fats contain mainly monounsaturated and saturated FAs. High amounts of branched and trans-FAs are observed in the samples of ruminants.
Permanent Link: http://hdl.handle.net/11104/0202210