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Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy

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    0367418 - BC 2012 RIV US eng J - Journal Article
    Vancová, Marie - Šlouf, Miroslav - Langhans, Jan - Pavlová, Eva - Nebesářová, Jana
    Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy.
    Microscopy and Microanalysis. Roč. 17, č. 5 (2011), s. 810-816. ISSN 1431-9276. E-ISSN 1435-8115
    R&D Projects: GA AV ČR KAN200520704; GA AV ČR KJB600960906; GA ČR GAP205/10/0348
    Institutional research plan: CEZ:AV0Z60220518; CEZ:AV0Z40500505
    Keywords : electron microscopy * colloidal palladium * nanoparticles * labeling * salivary glands * Ixodes ricinus
    Subject RIV: CF - Physical ; Theoretical Chemistry
    Impact factor: 3.007, year: 2011

    The application of palladium nanoparticles as electron-dense markers for labeling in both transmission and scanning electron microscopy requires their conjugation to a specific protein. The conjugation protocol described here includes the dihydrolipoic acid capping of Pd nanoparticles and their subsequent covalent attachment to protein molecules such as streptavidin, protein A, or avidin. The single-step reaction was mediated using the cross-linking agent ethyl-3-(3- dimethylaminopropyl)carbodiimide. The final Pd conjugates were fully functional, as demonstrated by labeling of ultrathin resin sections of either bovine serum albumin or secretory granules of the salivary gland isolated from the partially fed female Ixodes ricinus tick. The results were quantified, evaluated, and compared with results obtained using commercially available gold particle conjugates. Both the streptavidin-Pd conjugate and commercial streptavidin-Au conjugate were used to detect fucosylated glycans.
    Permanent Link: http://hdl.handle.net/11104/0202116

     
     
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