Difficulties with conventional phytoplasma diagnostic using PCR/RFLP analyses

0367362 - BC 2012 RIV IT eng J - Journal Article
Fránová, Jana
Difficulties with conventional phytoplasma diagnostic using PCR/RFLP analyses.
Bulletin of Insectology. Roč. 64, Suppl. (2011), s. 287-288. ISSN 1721-8861. E-ISSN 2283-0332
R&D Projects: GA MŠMT OC09021; GA AV ČR(CZ) 1QS500510558; GA MZe QH71145
Institutional research plan: CEZ:AV0Z50510513
Keywords : DNA extraction * PCR * primers * RFLP
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.592, year: 2011

Polymerase chain reaction (PCR) with subsequent restriction fragment length polymorphism (RFLP) analysis is often used for phytoplasma identification and classification. Although these techniques are very sensitive and specific, in some cases, nonspecific reactions, false positives and negatives results as well as unusual or illegible profiles after RFLP analyses, amplification of plant host´s DNA or other difficulties occurred. Experiences with suitability of positive and negative controls integration in PCR, evaluation of critical samples and other difficulties in phytoplasma PCR/RFLP identification are reported.
Permanent Link: http://hdl.handle.net/11104/0202071