Universal primers for plasmid detection and method for their relative quantification in phytoplasma-infected plants

0367361 - BC 2012 RIV IT eng J - Journal Article
Petrzik, Karel - Sarkisova, Tatiana - Čurnová, L.
Universal primers for plasmid detection and method for their relative quantification in phytoplasma-infected plants.
Bulletin of Insectology. Roč. 64, Suppl. (2011), s. 25-26. ISSN 1721-8861. E-ISSN 2283-0332
R&D Projects: GA MZe QH91224
Institutional research plan: CEZ:AV0Z50510513
Keywords : Plasmid * universal primers * rep gene
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.592, year: 2011

Rep gene occurring in the majority of plasmids associated with phytoplasmas was selected as a target for real-time PCR together with single-copy Tuf gene from the phytoplasma genome. Rep-specific primers amplified products from host plants infected with phytoplasmas of groups 16SrI, -II, -III, -V, -X, and -XII. Relative concentrations of plasmids ranged depending on strain from 1-2 copies to several hundred copies per each phytoplasma studied. Primers targeting the rep gene are proposed for direct PCR detection of phytoplasma plasmids from experimental periwinkle host as well as from natural plant hosts.
Permanent Link: http://hdl.handle.net/11104/0202070